Fig. 5

Direct target and the distribution of HCMV-miR-UL59 in exosomes. a The predicted binding site for hcmv-miR-UL59 in the 3′UTR of ULBP1 mRNA; b Western blotting analysis showed that the protein level of ULBP1 was downregulated in HEK293 cells transfected with hcmv-miR-UL59 mimics; c Real-time qPCR analysis showed ULBP1 mRNA level in HEK293 cells was not affected by hcmv-miR-UL59 overexpression; d Hcmv-miR-UL59 downregulated luciferase activity in HEK293 cells transfected with a pMIR-report plasmid that contained the 3′UTR of ULBP1 including HCMV-miR-UL59 binding site, whereas luciferase activity was not altered in cells transfected with MUT-ULBP1-3′UTR; e Absolute concentration of hcmv-miR-UL59 in plasma and exosome portions from 20 OLP patients using RT-qPCR analysis. *P < 0.05; **P < 0.01; ***P < 0.001