Fig. 4

a Cumulative population doublings of ASC expanded by cell culture media containing 5% (vol/vol) SRGF pool—batch A or SRGF pool—batch B. ASC were derived from n = 2 separate patients and were both expanded taking advantage of each different culture medium. No statistical differences were observed. Data of each curve are presented as means of cPD characterizing ASC from both patients. Error bars represents SD. Dots in the graph represent cell passages. b Images of induced (Stimulated) and non induced (Unstimulated) differentiation into adipogenic (Ad), chondrogenic (Ch) and osteogenic (Os) lineages of ASC expanded in presence of 5% (vol/vol) SRGF pool—batch A or SRGF pool—batch B. Differentiated cells were stained by Oil Red-O, Safranin-O and Alizarin Red, respectively