Fig. 2

Graphical representation of output results obtained by calculations performed by the statistical algorithm. The algorithm was programmed to create 500 statistical groups of random data from single donor SRGF and to repeat this operation by increasing the group statistical sample size step by step from n = 2 to n = 20. Thereafter, in created groups (n = 9500), the software calculated means for each growth factor and, matching groups characterized by the same sample size, the software retrieved the percent coefficient of variation [CV (%)] between calculated means. Calculated CV values in relation with statistical group size (n) were displayed in dot plots on Y-axis and X-axis, respectively. Solid line represents the selected CV threshold value and the dotted line represents the group statistical sample size (n = 16) required to maintain CV below the threshold for all analyzed growth factors