Fig. 2

Intracellular ROS assay in U937 cells simultaneously infected with Mycoplasma (M.F.) and treated with GYY4137 at two different time points (72 h and 5 days). The DCF fluorescence intensity is proportional to the ROS levels within the cell cytosol. Bars denote the standard deviation. The histograms shown are representative of data from three different experiments, with samples analyzed in duplicate. The p values were calculated as unequal variance t test of Mycoplasma infected cells relative to non-infected cells used as control (*p ≤ 0.05) and as unequal variance t test of Mycoplasma infected cells relative to Mycoplasma infected cells treated with GYY4137 (**p = 0.002, *p ≤ 0.05)