Fig. 1

a Hierarchal clustering of all 47 samples based on the rlog value [48]. The color density at the top right panel reflects the Euclidean distance. P CFS patients, C healthy controls. b Output of variation removal of our RNA-Seq data using RUVSeq. principle component analysis (PCA) is performed without using any differently expressed genes, and demonstrates relatively good separation between CFS patients (orange) and healthy controls (green). c Relative log expression (RLE) plot shows the distribution of read counts across all samples centered around zero. The y axis corresponds to the deviation of each RLE per gene per sample compared to median RLE over all samples (x axis). (CFS patients orange. Healthy controls green)