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Table 3 Analysis of HER2 protein levels in a patient subset

From: Her2 assessment using quantitative reverse transcriptase polymerase chain reaction reliably identifies Her2 overexpression without amplification in breast cancer cases

Patient or control

IHC

FISH

Copy number

mRNA

Protein expression

MCF10

Neg

Neg

Neg

Neg

Neg

SKBR3

Pos

Pos

Pos

Pos

Pos

p1

Neg

Neg

Neg

Neg

Neg

p2

Neg

Neg

Neg

Neg

Neg

p3

Equivocal (2+)

Neg

Neg

Neg

Neg

p4

Pos

Pos

Pos

Pos

Pos

p5

Equivocal (2+)

Equivocal

Neg

Neg

Neg

p6

Equivocal (2+)

Equivocal

Neg

Neg

Neg

p7

Equivocal (2+)

Equivocal

Neg

Neg

Neg

p8

Neg

Equivocal

Neg

Pos

Pos

p9

Equivocal (2+)

Equivocal

Neg

Pos

Pos

p10

Equivocal (2+)

Equivocal

Neg

Pos

Pos

p11

Equivocal (2+)

Neg

Neg

Pos

Pos

p12

Equivocal (2+)

Neg

Neg

Pos

Pos

  1. HER2 human epidermal growth factor receptor 2, IHC immunohistochemistry, FISH fluorescence in situ hybridization, mRNA messenger ribonucleic acid, neg negative, pos positive