Fig. 6

Immunofluorescence staining for TREM-1, TREM-2, CD86, CD206, CCR7, CD163, IFNγ, IL-4, CCR2 and CCR5 in the isolated CD14⁺ positive cells from the biopsy samples of obese non-diabetics and obese diabetics. Dual fluorescence staining was done using secondary antibody Alexa 488-Green for anti-human TREM-2, CD86, IFNγ, CCR5 and CD163 and Alexa 594-red for anti-human TREM-1, CD206, IL4, CCR2 and CCR7, counterstained with DAPI. Negative controls were run by using isotypes for each fluorochrome. A Immunofluorescence staining in the isolated CD14⁺ positive cells from the omentum biopsy samples for co-localization of TREM-1 and TREM-2 (Aa, Ab), co-localization of CD88 and CD206 (Ad, Ae), co-localization of IFNγ and IL4 (Ag, Ah), co-localization of CCR2 and CCR5 (Aj, Ak) and co-localization of CD163 and CCR7 (Am, An). B Immunofluorescence staining in the isolated CD14⁺ positive cells from the subcutaneous biopsy samples for co-localization of TREM-1 and TREM-2 (Ba, Bb), co-localization of CD88 and CD206 (Bd, Be), co-localization of IFNγ and IL4 (Bg, Bh), co-localization of CCR2 and CCR5 (Bj, Bk) and co-localization of CD163 and CCR7 (Bm, Bn). C Immunofluorescence staining in the isolated CD14⁺ positive cells from the liver biopsy samples for co-localization of TREM-1 and TREM-2 (Ca, Cb), co-localization of CD88 and CD206 (Cd, Ce), co-localization of IFNγ and IL4 (Cg, Ch), co-localization of CCR2 and CCR5 (Cj, Ck) and co-localization of CD163 and CCR7 (Cm, Cn). We found significantly increased immue reactivity of TREM-1, CD86, CCR7, IFNγ, CCR2 and CCR5 and decreased immune reactivity of TREM-2, CD163, CD206 and IL-4 in the isolated CD14⁺ positive cells from the biopsy samples of obese diabetics compared to obese non-diabetics (5 obese non-diabetics; 5 obese diabetics)