Detection of newly produced T and B lymphocytes by digital PCR in blood stored dry on nylon flocked swabs

Table 2 Values of TRECs and KRECs

Subject	Source of DNA	TRECs/mL qRT-PCR	TRECs/mL dPCR	KRECs/mL qRT-PCR	KRECs/mL dPCR
	PBMC^a	und	–	8294	–
#1	WB	und	261	17,636	16,684
	FS	und	74	17,183	15,041
	PBMC	3674	–	und	–
#2	WB	7854	6564	und	1312
	FS	7892	8488	und	1488
	PBMC	18	–	1136	–
#3	WB	252	462	4880	4048
	FS	und	106	2739	3580
	PBMC	149	–	6897	–
#4	WB	und	527	10,670	13,244
	FS	und	405	11,330	14,249
	PBMC	5220	–	7279	–
#5	WB	11,999	8231	22,300	17,112
	FS	4467	8046	17,494	15,230
	PBMC	5322	–	25,159	–
#6	WB	10,221	4824	41,282	32,753
	FS	4817	6403	31,934	28,184
	PBMC	9544	–	17,189	–
#7	WB	39,062	33,378	66,717	16,582
#7	FS	18,071	15,147	61,276	40,361
#8	PBMC	19,264	–	4357	–
	WB	27,926	23,385	7088	3096
	FS	27,828	22,671	3619	3959
#9	PBMC	24,809	–	23,333	–
	WB	25,778	25,811	20,599	17,346
	FS	24,563	20,244	26,541	16,852
#10	PBMC	30,817	–	22,099	–
	WB	52,494	38,190	42,171	32,453
	FS	41,428	35,845	42,037	35,649

dPCR digital PCR, FS flocked swabs, PBMC peripheral blood mononuclear cells, qRT-PCR quantitative real time PCR, und undetectable, WB whole blood
^aDNA from PBMC was analyzed with the “reference” method

ISSN: 1479-5876