Skip to main content

Table 2 Values of TRECs and KRECs

From: Detection of newly produced T and B lymphocytes by digital PCR in blood stored dry on nylon flocked swabs

Subject Source of DNA TRECs/mL qRT-PCR TRECs/mL dPCR KRECs/mL qRT-PCR KRECs/mL dPCR
  PBMCa und 8294
#1 WB und 261 17,636 16,684
FS und 74 17,183 15,041
PBMC 3674 und
#2 WB 7854 6564 und 1312
FS 7892 8488 und 1488
PBMC 18 1136
#3 WB 252 462 4880 4048
FS und 106 2739 3580
PBMC 149 6897
#4 WB und 527 10,670 13,244
FS und 405 11,330 14,249
PBMC 5220 7279
#5 WB 11,999 8231 22,300 17,112
FS 4467 8046 17,494 15,230
PBMC 5322 25,159
#6 WB 10,221 4824 41,282 32,753
FS 4817 6403 31,934 28,184
PBMC 9544 17,189
#7 WB 39,062 33,378 66,717 16,582
FS 18,071 15,147 61,276 40,361
#8 PBMC 19,264 4357
WB 27,926 23,385 7088 3096
FS 27,828 22,671 3619 3959
#9 PBMC 24,809 23,333
WB 25,778 25,811 20,599 17,346
FS 24,563 20,244 26,541 16,852
#10 PBMC 30,817 22,099
WB 52,494 38,190 42,171 32,453
FS 41,428 35,845 42,037 35,649
  1. dPCR digital PCR, FS flocked swabs, PBMC peripheral blood mononuclear cells, qRT-PCR quantitative real time PCR, und undetectable, WB whole blood
  2. aDNA from PBMC was analyzed with the “reference” method