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Table 1 Extraction efficiency of DNA from the flocked swabs impregnated with whole blood using three different extraction methods

From: Detection of newly produced T and B lymphocytes by digital PCR in blood stored dry on nylon flocked swabs

Extraction method

Elution volume (µL)

Mean DNA concentration (ng/µL)

Standard deviation (ng/µL)

Mean of total DNA yield (ng)

Standard deviation (ng)

QIAamp DNA Blood Mini Kit

25

18.00

1.74

450

87.18

Gentra Puregene Blood Kit

30

92.75

6.73

2783

201.80

Homemade protocol

40

121.07

8.53

4843

341.39

  1. Experiments were done in triplicate