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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Dynamic and specific immune responses against multiple tumor antigens were elicited in patients with hepatocellular carcinoma after cell-based immunotherapy

Fig. 2

The characteristics of mature DCs and activated T cells served in MASCT treatment. a The cellular uptake of peptides by immature DCs. Human monocyte-derived immature DCs were pulsed with fluorescent-labeled peptide of survivin (green, 2.5 μg/mL) for 2 h, followed by labeling with DAPI (blue) and lysotracker (red) to identify the nuclei and lysosomes, respectively. Fluorescent images were recorded by confocal microscopy, and the images are representative of four independent experiments. The scale bar is 7.5 μm. b The intracellular production of IFNγ, TNFα and granzyme B in the subsets of CD3+CD8+ T cells, CD3+CD4+ T cells, and CD3+CD56+ T cells. Activated T cells generated from HCC patients were stimulated with PMA for 4 h before being examined by flow cytometry. c Pie charts displaying the percentage of T cell subsets that co-expressed cytokines and enzymes. The mean ± SEM was shown. Triple producers: blue; double producers: gray; single producers: dark; non-producer: white. d Activated T cells generated from HLA-A2+ patients exhibited greater cytotoxic activity against the HCC cell line HepG2 (HLA-A2+) than HuH-7 cells (HLA-A2) at different E:T ratios (the ratio of effector cells to target cells), such as 40:1, 20:1, 10:1, and 5:1

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