Table 4 Comparison of the portion of CD4+ and CD8+ cells in CAR T cells manufactured from PBMC concentrates enriched for lymphocytes using 3 different methods
From: Elutriated lymphocytes for manufacturing chimeric antigen receptor T cells
Lymphocyte enrichment method | CAR T cell type | |||||
|---|---|---|---|---|---|---|
CD19 | GD2 | |||||
n | CD4 | CD8 | n | CD4 | CD8 | |
Anti-CD3/CD28 beads | 6 | 54.5 ± 26.1 | 35.0 ± 25.1 | 6 | 45.8 ± 12.3 | 48.4 ± 14.7 |
Anti-CD3/CD28 beads + plastic adherence | 16 | 57.3 ± 15.7 | 38.4 ± 14.8 | 5 | 48.0 ± 48.7 | 40.0 ± 10.1 |
Elutriation | 8 | 50.6 ± 14.8 | 42.4 ± 16.5 | 5 | 38.1 ± 17.1 | 53.2 ± 15.1 |