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Table 3 Quantity of total nucleated cells, CD3+ cells and transduced CD3+ cells in the final CAR T cell products and CD4+ and CD8+ cell content of the final products

From: Elutriated lymphocytes for manufacturing chimeric antigen receptor T cells

Cell type CAR T cell type
CD19 (n = 8) GD2 (n = 5) All (n = 13)
Mean ± SD Range Mean ± SD Range Mean ± SD Range
TNC (×106) 981 ± 235 668–1440 4384 ± 1194a 2250–6610 2289 ± 1865 668–6160
CD3+ cells (×106) 946 ± 273 500–1434 4119 ± 934a 2499–5119 2166 ± 1133 500–5119
Transduced CD3+ cells (×106) 517 ± 223 150–763 1940 ± 775a 837–3018 1064 ± 877 150–3018
CD3+ cells (%) 95.4 ± 8.6 74.9–99.9 95.1 ± 6.0 83.1–98.7 95.3 ± 25.5 74.9–99.9
Transduced CD3+ cells (%) 52.8 ± 15.0 30.0–69.3 45.7 ± 12.0 33.5–63.7 50.1 ± 21.3 30.0–69.3
CD4+ cells (%) 50.6 ± 14.8 24.9–72.3 38.1 ± 17.2 14.1–57.1 45.8 ± 19.7 14.1–72.3
CD8+ cells (%) 42.4 ± 16.5 18.3–65.7 53.3 ± 15.2 35.3–74.1 45.6 ± 19.6 18.3–74.1
  1. ap < 0.05 for comparison of CD19- and GD2-CAR T cells