Table 3 Quantity of total nucleated cells, CD3+ cells and transduced CD3+ cells in the final CAR T cell products and CD4+ and CD8+ cell content of the final products
From: Elutriated lymphocytes for manufacturing chimeric antigen receptor T cells
Cell type | CAR T cell type | |||||
|---|---|---|---|---|---|---|
CD19 (n = 8) | GD2 (n = 5) | All (n = 13) | ||||
Mean ± SD | Range | Mean ± SD | Range | Mean ± SD | Range | |
TNC (×106) | 981 ± 235 | 668–1440 | 4384 ± 1194a | 2250–6610 | 2289 ± 1865 | 668–6160 |
CD3+ cells (×106) | 946 ± 273 | 500–1434 | 4119 ± 934a | 2499–5119 | 2166 ± 1133 | 500–5119 |
Transduced CD3+ cells (×106) | 517 ± 223 | 150–763 | 1940 ± 775a | 837–3018 | 1064 ± 877 | 150–3018 |
CD3+ cells (%) | 95.4 ± 8.6 | 74.9–99.9 | 95.1 ± 6.0 | 83.1–98.7 | 95.3 ± 25.5 | 74.9–99.9 |
Transduced CD3+ cells (%) | 52.8 ± 15.0 | 30.0–69.3 | 45.7 ± 12.0 | 33.5–63.7 | 50.1 ± 21.3 | 30.0–69.3 |
CD4+ cells (%) | 50.6 ± 14.8 | 24.9–72.3 | 38.1 ± 17.2 | 14.1–57.1 | 45.8 ± 19.7 | 14.1–72.3 |
CD8+ cells (%) | 42.4 ± 16.5 | 18.3–65.7 | 53.3 ± 15.2 | 35.3–74.1 | 45.6 ± 19.6 | 18.3–74.1 |