Fig. 2

Properties of endothelial and smooth muscle cells after selection. a Endothelial cells at second passage after enrichment by MACS separation reveal CD31- and vWF-positive staining and produce extracellular matrix (collagen IV- and fibronectin-positive staining) (b). c Smooth muscle cells at the fifth passage reveal αSMA- and SMMCH-positive staining and produce extracellular matrix elastin. Scale bar 100 μm