Fig. 5

Effects of Notch1 on cell proliferation and EMT in GC cells. a The targets of Notch1 signaling were examined by Western blot assay after Notch1 downregulation using a shRNA. b Suppression of Notch1 caused inhibition of proliferation in GC cells. c The migration ability of GC cells was reduced in Notch1-silenced cells. d Suppression of Notch1 induced cell apoptosis. e The expression of E-cadherin was increased in Notch1 knocked down GC cells, while in contrast, N-cadherin, vimentin, and Snail expression levels were decreased. f Overexpression of Notch1 decreased E-cadherin expression following luteolin treatment in Hs-746T cells, while increased vimentin and pAKT expression. g The inhibiting effect of luteolin on cell migration was reversed subsequent to Notch1 overexpressing in Hs-746T cells. h Co-IP of β-catenin and NICD in GC cells. The interaction between NICD and β-catenin was abrogated with luteolin treatment in vitro and in vivo. i Proposed molecular model for Notch and β-catenin crosstalk. Results are the means of three independent experiments. *P < 0.05, **P < 0.01