Fig. 4

Effects of luteolin on EMT and Notch signaling in GC cells. a The chemical structure of luteolin. b The protein levels of the EMT markers were assessed by Western blot analysis in GC cells treated with different concentrations of luteolin. Luteolin increased E-cadherin levels and significantly decreased N-cadherin, β-catenin, vimentin, and Snail levels. c Immunofluorescence analysis showed that β-catenin was decreased in GC cells upon luteolin treatment (Green β-catenin, Blue DAPI, 200×). d The expression of Notch1, cyclin-D1, and Hes-1 was examined by Western blot analysis in GC cells after treatment with luteolin. e Gray scale ratio of Notch signaling markers in GC cells. f The mRNA levels of Notch targets were evaluated by RT-PCR. Results are the means of three independent experiments. *P < 0.05, **P < 0.01