Fig. 4
From: COX-2 expression positively correlates with PD-L1 expression in human melanoma cells
Representative IHC (a, b, f, g) and matched immunofluorescent staining (c, d, e, h, i, l) patterns of FFPE primary (a, b, c, d, e) and not matched metastatic lymph node (f, g, h, i, l) of melanoma tumors with COX-2-specific mAb (clone CX-294) (a, c, f, h), PD-L1-specific mAb (clone SP-142) (b, d, g, i) and both COX-2- (clone CX-294) and PD-L1-specific mAbs (clone SP-142) (e, l). Immunofluorescent staining matches to squared field of IHC staining. COX-2 was detected by goat anti-mouse IgG dylight 488 (green). Immunofluorescent staining of PD-L1 was detected by goat anti-rabbit IgG dylight 594 (red). Nuclei were stained by DAPI (blue). Negative controls are provided in Additional file 1: Figure S1. Arrows indicate examples of positive cells. Tumor margin (dotted line) and magnification are indicated