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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: IRE1α-XBP1 pathway promotes melanoma progression by regulating IL-6/STAT3 signaling

Fig. 4

IRE1α-XBP1 pathway promotes melanoma cell proliferation by regulating IL-6/STAT3 signaling. a Immunoblotting analysis of the indicated proteins and b XBP1 splicing levels, as determined by real-time PCR in Mel-RMu cells transfected with plasmids pCMV-IRE1α (IRE1) or pCMV (ctrl.) and treated with anti-IL-6 antibodies (anti-IL-6). c Immunoblotting analysis of the indicated proteins in Mel-RMu cells transfected with plasmids pCMV-XBP1 (XBP1s) or pCMV (ctrl.) and treated with anti-IL-6 antibodies (anti-IL-6). d, e Cell proliferation was analyzed by CCK8 assay (d) and BrdU assay (e) in Mel-RMu cells transfected with plasmids pCMV-IRE1α (IRE1) or pCMV (ctrl.) and treated with anti-IL-6 antibodies (anti-IL-6). f, g Cell proliferation analyzed by CCK8 assay (f) and BrdU assay (g) in Mel-RMu cells transfected with plasmids pCMV-XBP1 (IRE1) or pCMV (ctrl.) and treated with anti-IL-6 antibodies (anti-IL-6). The results are from more than three independent experiments. The data are presented as the mean ± s.e.m. *P < 0.05, **P < 0.01 by Student’s t test, one-way or two-way ANOVA relative to the ctrl. group; # P < 0.05 by Student’s t-test relative to IRE1 + anti-IL6 or XBP1s + anti-IL6 groups, as indicated

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