Fig. 2
Histological analysis of skin morphology of wounds on day 14 after treatment with Ex-4, ADSCs, or combination of Ex-4 and ADSCs. Diabetic db/db mice were subjected to wounding (6 mm in diameter) by punch biopsy on the skin of the back. One day later, each wound received 2.5 × 105 ADSCs injected intradermally around the wound and/or 100 nM Ex-4 was applied to the wound with a fine brush, daily. a H&E staining of wound area (×400). b The thickness of the dermis observed through the H & E staining results. *p < 0.05 vs wound-(-), $ p < 0.05 vs wound-ADSC, # p < 0.05 vs wound-EX-4. c Masson’s trichrome staining of wound area (×400). c Masson’s trichrome staining of wound area (×400). d The collagen density observed through Masson’s trichrome staining results. *p < 0.05 vs wound-(−), $ p < 0.05 vs wound-ADSC, # p < 0.05 vs wound-EX-4. e Immunofluorescence staining of VEGF. (×800). f VEGF expression levels observed through VEGF staining results. *p < 0.05 vs vehicle, # p < 0.05 vs EX-4