Table 1 Primary antibodies used for immunohystochemical reactions
From: Bone Morphogenetic Proteins and myostatin pathways: key mediator of human sarcopenia
Antibody | Characteristics | Dilution | Retrieval |
|---|---|---|---|
Anti-Pax7 | Rabbit monoclonal, clone NC, Novus Biologicals | 1:100 | Citrate pH 6.0 |
Anti-myogenin | Rabbit monoclonal, clone F5D, AbCam Cambridge, UK | 1:100 | EDTA citrate pH 8.0 |
Anti-BMP-2 | Rabbit clone N/A; Novus Biologicals, Littleton, CO, USA | 1:100 | Citrate pH 6.0 |
Anti-BMP-4 | Rabbit polyclonal clone NBP1-91805; Novus Biologicals, Littleton, CO, USA | 1:500 | Citrate pH 6.0 |
Anti-BMP-7 | Mouse monoclonal clone ab54904; AbCam, Cambridge, UK | 1:250 | Citrate pH 6.0 |
Anti-myostatin | Rabbit monoclonal, clone ab134682, AbCam, Cambridge, UK | 1:100 | EDTA citrate pH 8.0 |
Anti-Smad1-5-8 | Rabbit polyclonal, clone ab13723, AbCam, Cambridge, UK | 1:100 | EDTA citrate pH 8.0 |