Tacrolimus in the prevention of adverse pregnancy outcomes and diabetes-associated embryopathies in obese and diabetic mice

Table 2 Summary of the major skeletal malformations, variations and delayed ossifications in fetuses examined

	HFD-dNONcNZO	Tacrolimus (0.05 mg/kg)	Tacrolimus (0.1 mg/kg)	Metformin (200 mg/dL)
Fetuses (litters)	3 (3)	22 (3)	18 (3)	17 (3)
14 ribs	33% (1)	27% (3)	44% (3)	18% (2)
Incomplete ossification
Sternebra	^a	0%	0%	6% (1)
Metacarpals	^a	0%	0%	6% (1)
Forelimb phalanges	33% (1)	0%	6% (1)	12% (2)

Skeletons of viable fetuses were double-stained with Alcian blue and Alizarin red and assessed for malformations, variations and ossification delays. The incidences are reported in percentages with the values in parentheses reflecting the number of litters affected
^aLitters were all resorbed or dead. Therefore no visceral or skeletal examinations were done on them (only external examination because the numbers of pups was so low)

ISSN: 1479-5876