Fig. 2

Low fetal viability and high rate of fetal demise were characteristics of pregnancy in the HFD-dNONcNZO dams. a–c Findings at necropsy at gd18.5 for the common gross manifestations of GDM among the HFD-dNONcNZO dams typically expressing low fetal viability and higher rates of fetal resorption. Notwithstanding its effect on birth weight, treatment with tacrolimus enhanced live birth rate (d), lowered percentage of demise (e) and enhanced fetal/placental (F/P) weight ratio (f). Reference values for random blood glucose were also detected among pups delivered to tacrolimus-treated dams (g). n = a minimum of 7 dams/group with variable numbers of fetuses/pups examined. p < 0.001 in d