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Table 1 Protocol reproducibility with the optimized clinical-grade compatible conditions using buffy coats from eight donor pairs

From: Generation of donor-specific Tr1 cells to be used after kidney transplantation and definition of the timing of their in vivo infusion in the presence of immunosuppression

 

CD14+ purity (%)

DC-10 yield (%)

CD4+ purity (%)

T10-cell yield (%)

Donor-specific anergy (%)

Mean

95

13

98

53

80

SD

3

6

1

31

10

Range

90–99

4–21

97–99

17–123

63–94

CV

3.3

47.3

0.9

59.0

14.6

  1. DC-10 tolerogenic dendritic cells, SD standard deviation, CV coefficient of variation