Fig. 8
Freezing has little effect on donor-specific anergy and Tr1-cell content of T10 cells. T10 cells were thawed after 1, 6 or 12 months of freezing and immediately stimulated with donor-mDC. In parallel, flow cytometry staining for Tr1 cells was performed. Percentages of donor-specific anergy (a) and Tr1 cell content (b) are shown. One symbol represents one T10 cell preparation starting from buffy coats from healthy donors. Lines represent the time course of the percentage of anergy or Tr1-cell content of individual T10 cells thawed at different timepoints. Dotted line represents the set cutoff anergy level of 60%. Tr1-cell content 12 months after freezing was not available