Fig. 6

Donor-specific suppression of CD4⁺ T cell proliferation by T₁₀ cells correlates with Tr1-cell content. CD4⁺ T cells were stimulated with donor mDC (or third party mDC—when numbers of T₁₀ cells permitted) in the presence or absence of autologous T₁₀ cells. Percentages of suppression by T₁₀ cells are shown. One dot represents one experiment with buffy coats from healthy donors (each symbol depicts one donor and different shapes represent different donor pairs). Lines represent mean value of each data set ±SD (*p ≤ 0.05 Wilcoxon matched pairs test) (a). Percentages of Tr1-cell content within T₁₀ cells is plotted against percentage of suppression by T₁₀ cells. One dot represents one experiment. Numbers represent anergy levels measured on each T₁₀-cell preparation. Line represents linear regression (b)