Fig. 1
Graphical representation of the protocol for generating T10 cells to be used in kidney transplanted patients. CD14+ cells are selected from the kidney donor leukapheresis and cultured with GM-CSF and IL-4 in the presence (DC-10) or absence (mDC) of IL-10. At day 5, mDC are activated with monophosphoril MPL. Upon harvest at day 7 of culture, DC-10 and mDC are irradiated and co-cultured for 10 days with CD4+ T cells selected from the recipient leukapheresis and exogenous IL-10 to generate T10 cells. mDC cultured with CD4+ T cells without IL-10 generate the control Tm cell population. T10 cells are donor-specific anergic CD4+ T cells, yet they respond to third party mDC stimulation (IIIp mDC) similar to the control population as shown by the representative cell-proliferation bars (simulating what one should expect when measuring T10 cell proliferation toward donor mDC or toward third party unrelated mDC)