Fig. 5

Selectively increased expression of HO-1 in splenic CD4⁺ T cells. a mRNA expression of HO-1 in splenic CD4⁺ T cells and non-CD4⁺ splenocytes in each group of mice (n = 5/each group). Values are shown mean ± SD. *P < 0.05. b Mouse splenic CD4⁺ T cells and non-CD4⁺ splenocytes were isolated from each group of mice. Lysates were prepared and analyzed by western blotting to detect HO-1. HO-1 expression was higher in a dose-dependent manner in splenic CD4⁺ T cells from A77 1726-treated mice compared with cells from vehicle-treated mice. c Representative images of HO-1-positive cells (stained in brown; original magnification ×400) are shown (left). The results are quantified as the mean ± SD for the number of HO-1-positive cells (n = 3 mice per group (right). *P < 0.05; **P < 0.01; ***P < 0.001