Fig. 3

Effects of A77 1726 on inflammatory molecules and oxidative stress in the joints of IL-1Ra-KO mice. a Tissue sections from the joints of IL-1Ra-KO mice treated with A77 1726 or vehicle were stained with antibodies to TNF-α, IL-1β, iNOS, or nitrotyrosine. The cells stained with each antibody are shown in brown (left). Expression of proinflammatory molecules and iNOS and nitrotyrosine (oxidative stress markers) was significantly attenuated in the joints of A77 1726-treated IL-1Ra-KO mice compared with vehicle-treated animals (right). b Levels of circulating TNF-α, IL-1β and MDA in the serum of IL-1Ra-KO mice in each group. Values are shown as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001