Skip to main content
Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Adjuvant effects of a sequence-engineered mRNA vaccine: translational profiling demonstrates similar human and murine innate response

Fig. 5

mRNA vaccine induced multiple genes of innate immunity in the dLN. RNA was isolated from RNAlater-preserved LN biopsies (axillar and brachial) collected 6 or 24 h post ID application of mRNA vaccine. Whole genome expression analysis was performed using Affymetrix WT Mouse Gene-2.1-ST GeneChip Array. a Heat map represents normalized mean expression values depicted as log2 fold change relative to the respective buffer control. Transcripts were grouped by their immune function. b, c dLN (axillar, brachial and inguinal) were harvested 24 h post treatment and single cell suspensions were analyzed using polychromatic flow cytometry gating on live cells by followed by staining for markers of (b) migratory dendritic cells (CD11c+MHCIIhigh cells) and (c) B cells (B220+CD19+ cells). The surface expression of CD86 on dendritic cells and B cells was determined as geometric mean fluorescence intensity (GMFI). Mean ± SEM are shown for n = 8 mice per group. Statistical analysis was performed with GraphPad Prism software (version 5.04) using Kruskal–Wallis test followed by Dunns multiple comparison test. p value indicators ***, **, * refer to p < 0.05, p < 0.01 and p < 0.001, respectively

Back to article page