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Table 5 CD45neg cell analysis in healthy blood samples

From: Advances in rare cell isolation: an optimization and evaluation study

Sample type Sample Nr. Large cells (>10 µm) Medium cells (7–10 µm) Small cells (4 > 6 µm) Sum of cells Blood resting time (h)
Buffy coat (n = 9) 1 2 1 0 3 24
2 1 0 0 1
3 2 2 0 4
4 1 0 0 1
5 2 4 0 6
6 0 0 0 0
7 4 4 2 10
8 1 1 0 2
9 0 0 0 0
  Average 1.4 1.3 0.2 3  
Whole blood sample: clean collection after 6 mL (n = 9) Donor 1 0 0 3 3 6
Donor 2a 0 0 16 16 6
Donor 3 1 2 0 3 6
Donor 4 1 1 2 4 6
Donor 5 0 4 2 6 6
Donor 6b 12 1 1 14 3
Donor 7 0 1 0 1 6
Donor 8 3 3 0 6 3
Donor 9c 6 9 7 22 1
Average 2.6 2.3 3.44 8.3  
Whole blood sample: discharge collection of the first 6 mL (n = 8) Donor 1 0 0 0 0 3
Donor 2a 1 1 8 10 3
Donor 3 3 1 0 4 3
Donor 4 1 1 3 5 3
Donor 5 N.A. N.A. N.A. N.A. 3
Donor 6b 4 0 0 4 6
Donor 7 1 1 0 2 3
Donor 8 3 2 0 5 6
Donor 9c 7 8 2 17 1
Average 2.5 1.8 1.6 5.9  
  1. aDonor 2, blood condition; threefold higher platelet count, low RBC count
  2. bDonor 6, under suspicion of abnormal cell content, followed up candidate
  3. cDonor 9, frequent blood donor (donation of 500 mL two weeks before the test)