Fig. 5
From: Advances in rare cell isolation: an optimization and evaluation study
Cell image analysis. Cells are recorded at 4 different appearances from left to right; under brightfield, AO fluorescence, PE fluorescence and contrast enhanced (ImageJ manual brightness adjustment) PE fluorescence. The bars represent a length of 10 µm. a CD45 positive cell identification. The cell indicated by a white arrow shows low AO as well as ring shaped PE fluorescence. CD45 negative cell identification with normal (b) and high (c) AO fluorescence intensity without ring formation in the light wavelength above 550 nm. Residual fluorescence is pronounced (b) and at noise level (c), visible only after contrast enhancement. The fluorescence signal is ascribed to AO fluorescence only