Fig. 3

AMD iPSC-RPE exhibit increased susceptibility to oxidative stress and produce higher ROS. a Cell viability assays of AMD and control iPSC-RPE treated with increasing concentrations of H₂O₂ for 48 h. Higher susceptibility to oxidative stress-induced cell death under oxidative stress conditions (0.1, 0.2 and 0.4 mM H₂O₂) is observed in AMD RPE-iPSC-RPE (9R, 32R) and AMD Skin-iPSC-RPE (005BF) as compared to normal RPE-iPSC-RPE (6R, 10R, 25R). b ROS production under stress conditions is significantly higher in AMD RPE-iPSC-RPE (9R, 32R) and AMD Skin-iPSC-RPE (005BF) as compared to normal RPE-iPSC-RPE (6R, 10R, 25R). Asterisk (*) in a and b indicates statistically significant difference between control and AMD iPSC- RPE, determined by student t test, p ≤ 0.05