Fig. 2

Result of iNOS expression on virus titer and tumor volume. a HCT-116 tumors were harvested 3, 7, 14 and 21 dpi and infectious viral particles were quantitated on CV-1 (n = 3–5 mice/time point/experiment). The bar graph shows the mean virus titer as PFU per gram of tumor for both LIVP 1.1.1 and GLV-1h68 as test versus control virus (GLV-1h68 is a recombinant isolate from the VACV LIVP strain, as described previously [41]), respectively. b An inverse relationship between the average tumor volume and the number of iNOS⁺ cells induced by LIVP 1.1.1. The plot represents the infiltration kinetics of iNOS⁺ cells (solid line) over change in tumor growth (bars) on days 7, 14 and 21. c iNOS expression was assessed as relative MFI using flow cytometry. Histogram shows relative MFI on gated iNOS⁺ PMN cells isolated from the tumor and spleen on day 7. d The change of iNOS MFI over time. All of the treatment groups have a significant difference from the control except for the day 21; day 7 tumor; p = 0.0174 and spleen; p = 0.0035 and day 14 tumor; p = 0.0079 and spleen; p = 0.0052). Each time point was repeated at least two times