Fig. 5
EMPs can stimulate TNF-α and IL-6 release in HUVECs, which can be down-regulated by P38-siRNAs. A, B Immunoblotting showed EMPs can increase P38 expression, which can be reduced by siRNAs targeting P38 MAPK (Si-P38). C, D TNF-α and IL-6 concentration were relative low in control (C) and negative (Neg) group. After 6 h stimulated with EMPs, TNF-α and IL-6 level increased in each group. TNF-α and IL-6 level in siRNAs P38 MAPK group was significantly lower than that in non-P38 siRNAs interfering groups. (*P < 0.05 compared with control group, **P < 0.05 compared with negative group, ***P < 0.05 compared with the group of EMPs 105/mL, n = 8)