Performance evaluation of the touchscreen-based Muse™ Auto CD4/CD4% single-platform system for CD4 T cell numeration in absolute number and in percentage using blood samples from children and adult patients living in the Central African Republic

Table 3 CD4 T cell counting in absolute count and percentage in 150 HIV-1-infected patients living in Central African Republic, by the single-platform Muse™ Auto CD4/CD4% system and by the BD FACSCount system, at various CD4 T cell count ranges according to the BD FACSCount system results

Categories	<200 cells/µl	200–350 cells/µl	>350 cells/µl
Number	30	35	85
Absolute CD4 T cells (cells/µl)
Muse™ Auto CD4/CD4% system
Mean ± SD	90 ± 106	276 ± 13	1225 ± 1082
BD FACSCount system
Mean ± SD	79 ± 107	273 ± 4	1281 ± 844
Absolute bias (limits of agreement)^a
Mean (±1.96 SD)	+9.5 (+2.6; +16.4)	+11.5 (+4.6; +18.4)	−73.0 (−491.5; +345.1)
Percent CD4 T cells (%CD4)
Muse™ Auto CD4/CD4% system
Mean ± SD	10.0 ± 12.7	20.0 ± 5.7	32.0 ± 2.8
BD FACSCount system
Mean ± SD	8.5 ± 12.0	16.0 ± 2.8	28.0 ± 2.9
Absolute bias (limits of agreement)^a
Mean (±1.96 SD)	+0.5 (−0.8; +1.9)	+3.0 (−5.3; +11.2)	+1.0 (−7.3; +9.3)

SD standard deviation
^aThe Bland–Altman analysis was carried out to calculate the absolute bias and limits of agreement which are the 95% confidence intervals (±1.96 × SD) of the mean bias of all paired measurements in a given category [27]

ISSN: 1479-5876