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Table 3 CD4 T cell counting in absolute count and percentage in 150 HIV-1-infected patients living in Central African Republic, by the single-platform Muse™ Auto CD4/CD4% system and by the BD FACSCount system, at various CD4 T cell count ranges according to the BD FACSCount system results

From: Performance evaluation of the touchscreen-based Muse™ Auto CD4/CD4% single-platform system for CD4 T cell numeration in absolute number and in percentage using blood samples from children and adult patients living in the Central African Republic

Categories <200 cells/µl 200–350 cells/µl >350 cells/µl
Number 30 35 85
Absolute CD4 T cells (cells/µl)
 Muse™ Auto CD4/CD4% system
  Mean ± SD 90 ± 106 276 ± 13 1225 ± 1082
BD FACSCount system
  Mean ± SD 79 ± 107 273 ± 4 1281 ± 844
 Absolute bias (limits of agreement)a
  Mean (±1.96 SD) +9.5 (+2.6; +16.4) +11.5 (+4.6; +18.4) −73.0 (−491.5; +345.1)
Percent CD4 T cells (%CD4)
 Muse™ Auto CD4/CD4% system
  Mean ± SD 10.0 ± 12.7 20.0 ± 5.7 32.0 ± 2.8
 BD FACSCount system
  Mean ± SD 8.5 ± 12.0 16.0 ± 2.8 28.0 ± 2.9
 Absolute bias (limits of agreement)a
  Mean (±1.96 SD) +0.5 (−0.8; +1.9) +3.0 (−5.3; +11.2) +1.0 (−7.3; +9.3)
  1. SD standard deviation
  2. aThe Bland–Altman analysis was carried out to calculate the absolute bias and limits of agreement which are the 95% confidence intervals (±1.96 × SD) of the mean bias of all paired measurements in a given category [27]