Table 1 Cell yields. Number of cells seeded and final number of cells obtained after ASC expansion P0 and P1 in flasks and Quantum System
n = 3 | SVF seeded | SVF seeded per cm2 | Mean days in culture | ASC P0 harvested | Viability | ASC:SVF ratio |
|---|---|---|---|---|---|---|
Flask (T75) | 4.50 × 106 | 6.00 × 104 | 8 ± 1.41 | 2.37 ± 0.88 × 106 | 97% | 0.53 ± 0.01 |
Quantum | 5.56 ± 1.76 × 107 | 2.65 × 103 | 15 ± 8.54 | 8.93 ± 4.88 × 107 | 96% | 1.61 ± 0.01 |
n = 3 | ASC P0 | ASC seeded per cm2 | Mean days in culture | ASC P1 harvested | Viability | PD |
|---|---|---|---|---|---|---|
F–F | 3.50 × 105 | 4.67 × 103 | 17 ± 5.57 | 7.53 ± 0.77 × 105 | 98% | 1.10 ± 0.15 |
Q–Q | 2.10 × 107 | 1.00 × 103 | 18 ± 5.77 | 9.53 ± 0.58 × 107 | 96% | 2.18 ± 0.09 |
F–Q | 2.10 × 107 | 1.00 × 103 | 17 ± 6.03 | 9.91 ± 1.28 × 107 | 97% | 2.23 ± 0.19 |