Fig. 5
AQP5 expression in HL-60 promyelocytes after fMLP administration and in whole blood of septic patients. a AQP5 mRNA expression in HL-60 cells stimulated with 10−8 M fMLP for 6 h. FMLP significantly increased AQP5 expression (n = 5; p = 0.03). b AQP5 protein expression in fMLP stimulated HL-60 cells for 6 h. AQP5 protein amount is increased in fMLP (10−8 M)-stimulated cells (lane 2) compared to control (lane 1). c AQP5 RNA expression measured with RT-PCR in whole blood of septic patients (n = 14, 11); AA-genotype carriers showed an increased AQP5 mRNA expression compared to AC-genotype carriers (p = 0.025). No RNA was available from CC genotype carriers. d TNF-alpha serum concentrations of septic patients. No differences could be detected between genotypes (p ≫ 0.05); Data derive from serum of septic patients [(n = 65, 33, 3) a–d], data are shown as boxplots (min to max)