Fig. 2

Neutrophil migration in peritoneum and lung 3 h after LPS or saline injection i.p. (20 mg/kg). a Total cell count in the peritoneum was 1.2 * 10⁶ cells/ml in median in Aqp5-KO mice compared to 2.5 * 10⁶ cells/ml in WT mice (n = 8; p = 0.04). b Representative dot plots show flow cytometric analysis of CD11c and Ly6G/C positive cells in the peritoneum. CD11c Ly6G/C positive cells were increased in WT mice compared to Aqp5-KO mice after LPS injection c Percentage of CD11c Ly6G/C positive cells in the peritoneum. In WT mice (n = 4) percentage of CD11c Ly6G/C positive cells significantly increased after LPS injection compared to saline injection (p = 0.017) and was higher compared to Aqp5-KO mice (n = 4) (p = 0.035). d Total cell count of CD11c Ly6G/C positive cells (n = 8; p = 0.04). e Representative NASDCL-Esterase staining of formalin fixed lung tissues (40× magnification). WT mice (n = 2) showed higher number of neutrophils compared to Aqp5-KO (n = 2) mice (red arrows) after 3 h LPS injection f Myeloperoxidase (MPO) activity in mice lungs. In WT mice (n = 4) MPO activity significantly increased after LPS injection compared to saline (p = 0.04) and was higher compared to Aqp5-KO mice [n = 4 (p = 0.004) f]. Data are shown as boxplots (min to max; n = 8 in total)