Fig. 3

Outcomes of cells cultured with BAL fluid from mice with ARDS. a Proliferation of splenocytes from naïve mice cultured with BAL fluid from mice with ARDS (BAL, n = 5, experiments in triplicate) compared to cells cultured with BAL fluid where the sPD-1 was precipitated out (Anti-sPD-1, n = 5, experiments in triplicate) Analysis done by t test, *p = 0.032 when compared to cells cultured with BAL fluid where sPD-1 was precipitated out. b TNF-α levels of splenocytes from naïve mice cultured with BAL fluid from mice with ARDS (BAL, n = 5) compared to cells cultured with BAL fluid where the sPD-1 was precipitated out (Anti-sPD-1, n = 5) Analysis done by t test, *p = 0.002 when compared to cells cultured with BAL fluid from mice with ARDS. c IL-6 levels of splenocytes from naïve mice cultured with BAL fluid from mice with ARDS (BAL, n = 5) compared to cells cultured with BAL fluid where the sPD-1 was precipitated out (Anti-sPD-1, n = 5)