Fig. 8
From: An ex vivo human cartilage repair model to evaluate the potency of a cartilage cell transplant
Aggrecan protein expression in 2 week old spheroids before implantation. Immunohistochemical detection of ACAN protein expression shown as a red staining (arrowheads) in representative spheroid samples before implantation. a High ACAN protein expression in spheroids of donor #8, b lower expression predominantly in the outer layers of spheroids of donor #10 and c absence of ACAN expression in spheroids of donor #14. d IgG isotype control. Sections were counterstained with haematoxylin, staining the nuclei blue. Scale bar: 100 µm