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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Mesenchymal stromal cells derived from cervical cancer produce high amounts of adenosine to suppress cytotoxic T lymphocyte functions

Fig. 5

Adenosine generated by CeCa-MSCs strongly inhibits CD8+ T-cell activation. A total of 5 × 105 CD8+ T-cells obtained by negative selection were cultured with beads containing anti-CD2/CD3/CD28 antibodies in a 2:1 ratio and in the presence of supernatants (Sup) obtained from NCx-MSC or CeCa-MSC cultures. Some cells were cultured in the presence of 300 μM caffeine (CAF), a nonselective antagonist of Ado receptors, or 1 µM ZM241385 (ZM), a selective antagonist of A2A receptor, and combinations of these antagonists, as indicated. CD8+ T-cells cultured in the presence of 500 μM synthetic Ado were used as positive controls of inhibition. After 96 h of culture, the CD8+IFN-γ+ T-cell percentage was determined. Significant differences *(P < 0.05) and **(P < 0.01) were obtained compared with CD8+ T-cells cultured with beads in the absence of Ado (negative control of inhibition). Data are representative of three independent experiments, and the mean ± SEM are shown

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