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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Mesenchymal stromal cells derived from cervical cancer produce high amounts of adenosine to suppress cytotoxic T lymphocyte functions

Fig. 1

Functional characterization of mesenchymal stromal cells (MSCs) from normal cervix (NCx-MSCs) and cervical cancer (CeCa-MSCs). A Immunophenotype of MSCs cells. Cells were stained with FITC, PE or APC-conjugated monoclonal antibodies against surface markers indicated or immunoglobulin isotype control antibodies. Cells were analyzed using flow cytometry. Representative histograms for the indicated cell markers are shown. B MSCs from the two sources (NCx and CeCa) were cultured in the adipogenic, osteogenic and chondrogenic induction medium for 14, 21, and 28 days, respectively. (a) and (d) Adipogenic differentiation was indicated by accumulation of neutral lipid vacuoles that stained with Oil Red O. Scale bar 100 µm (magnification ×20). (b) and (e) Osteogenic differentiation was indicated with alkaline phosphatase staining. Scale bar 100 µm (magnification ×20). (c) and (f) Chondrogenic differentiation was indicated by the chondrogenic matrix colored by Alcian blue in cryosections from pelleted micromass. Scale bar 100 µm (magnification ×20). One representative experiment is showed

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