Fig. 3

ERCs promoted hepatic cell proliferation and suppressed inflammatory cell infiltration after CCl₄ injury. Immunohistochemical staining for PCNA and Ly6G were carried out as previous described. PCNA and Ly6G staining of liver sections in the mice with or without ERC administration were performed 24 h after CCl₄ treatment. The untreated group showed relatively few PCNA⁺ hepatocytes and abundant Ly6G⁺ cells in centrilobular areas. Sections from ERCs treated group exhibited numerous PCNA⁺ hepatocytes surrounding the edge of hepatocellular necrosis and fewer inflammatory cell accumulating in liver tissues. The numbers of PCNA⁺ and Ly6G⁺ cells in the liver sections were measured. At least six 12 mm² tissue sections were counted for each mouse. Values represent mean ± SEM. (^## p < 0.01 versus the normal control group. *p < 0.05, **p < 0.01 versus the untreated group, n = 6). (Magnification 100×)