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Table 2 Prediction results for the investigated HAdV epitope candidates

From: Discovery of immunodominant T-cell epitopes reveals penton protein as a second immunodominant target in human adenovirus infection

Abbreviation Prediction score
SYFPEITHI [score] BIMAS [rank] NetChop
NetMHCcons [BL]b NetMHC [BL]b NetMHCstab [SL]c, T a1/2
A01HexonTDLG 20 0.125 [134] 11052.58 13301 0.63
A01HexonTNDQ 28 6.25 [11] 677.84 [SB] 1182 2.08 [WS]
A01HexonQNDP 27 125 [1] 1691.19 [WB] 3899 2.97 [WS]
A02Hexon TLLY 27 3432.948 [1] 3.25 [SB] 3 [SB] 14.1 [HS]
A02HexonTLAV 25 69.552 [15] 649.13 513 1.09
B08HexonGLRY 32 160 [1] 42.02 [SB] 40 [SB]
B08Hexon DLQD 26 24 [2] 2339.71 3791
A01Penton STDV 35 312.5 [1] 5.9 [SB] 7 [SB] 4.86 [WS]
A01PentonSNDS 28 6.25 [8] 953.12 [WB] 2784 1.74
A01PentonSSDI 33 187.5 [1] 8.07 [SB] 9 [SB] 4.23 [WS]
A01PentonLTDH 21 6.25 [9] 43.65 [SB] 30 [SB] 1
A02Penton ILHT 23 271.948 [2] 16.75 [SB] 18 [SB] 5.72 [WS]
A02PentonALGI 23 69.552 [5] 69.13 [WB] 64 [WB] 7.77 [HS]
A02PentonGNIP 20 >20 16673.41 17051 0.5
A03PentonVLES 25 9 [4] 1073.58 1099 0.7
A03PentonLLPG 23 3 [5] 27426.93 21324 0.22
B08PentonNTKY 29 80 [2] 39.6 [SB] 26 [SB]
B08Penton DSKG 28 160 [1] 755.3 [WB] 799
B08PentonLTKD 32 120 [2] 2134.13 4050
B08Penton DSKK 28 160 [1] 130.88 [SB] 80 [WB]
  1. Prediction results for 19 epitope candidates (plus one reference epitope, A01HexonTDLG) predicted for frequent HLA class I alleles and clinically relevant HAdV types using three different prediction tools (SYFPETHI, BIMAS, and NetChop). Epitope candidates were selected with respect to their predicted HLA binding affinity (BL binding level) and peptide-HLA complex stability (SL stability level). Epitopes were classified as weak binders (WB threshold 500 nM) or strong binders (SB threshold 50 nM) with either weak stability (WS threshold 2 h) or high stability (HS threshold 6 h) according to the predicted scores.
  2. Pre-classified immunodominant peptide candidates are highlighted in italic
  3. aEstimated half-time (t1/2) of dissociation (in hours)
  4. bEstimated peptide binding affinity to HLA alleles as IC50 value (in nM)
  5. cEstimated stability of peptide-MHC I complexes as IC50 value (in nM), and IC50 = half-maximum inhibitory concentration