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Table 2 Prediction results for the investigated HAdV epitope candidates

From: Discovery of immunodominant T-cell epitopes reveals penton protein as a second immunodominant target in human adenovirus infection

Abbreviation

Prediction score

SYFPEITHI [score]

BIMAS [rank]

NetChop

NetMHCcons [BL]b

NetMHC [BL]b

NetMHCstab [SL]c, T a1/2

A01HexonTDLG

20

0.125 [134]

11052.58

13301

0.63

A01HexonTNDQ

28

6.25 [11]

677.84 [SB]

1182

2.08 [WS]

A01HexonQNDP

27

125 [1]

1691.19 [WB]

3899

2.97 [WS]

A02Hexon TLLY

27

3432.948 [1]

3.25 [SB]

3 [SB]

14.1 [HS]

A02HexonTLAV

25

69.552 [15]

649.13

513

1.09

B08HexonGLRY

32

160 [1]

42.02 [SB]

40 [SB]

–

B08Hexon DLQD

26

24 [2]

2339.71

3791

–

A01Penton STDV

35

312.5 [1]

5.9 [SB]

7 [SB]

4.86 [WS]

A01PentonSNDS

28

6.25 [8]

953.12 [WB]

2784

1.74

A01PentonSSDI

33

187.5 [1]

8.07 [SB]

9 [SB]

4.23 [WS]

A01PentonLTDH

21

6.25 [9]

43.65 [SB]

30 [SB]

1

A02Penton ILHT

23

271.948 [2]

16.75 [SB]

18 [SB]

5.72 [WS]

A02PentonALGI

23

69.552 [5]

69.13 [WB]

64 [WB]

7.77 [HS]

A02PentonGNIP

20

>20

16673.41

17051

0.5

A03PentonVLES

25

9 [4]

1073.58

1099

0.7

A03PentonLLPG

23

3 [5]

27426.93

21324

0.22

B08PentonNTKY

29

80 [2]

39.6 [SB]

26 [SB]

–

B08Penton DSKG

28

160 [1]

755.3 [WB]

799

–

B08PentonLTKD

32

120 [2]

2134.13

4050

–

B08Penton DSKK

28

160 [1]

130.88 [SB]

80 [WB]

–

  1. Prediction results for 19 epitope candidates (plus one reference epitope, A01HexonTDLG) predicted for frequent HLA class I alleles and clinically relevant HAdV types using three different prediction tools (SYFPETHI, BIMAS, and NetChop). Epitope candidates were selected with respect to their predicted HLA binding affinity (BL binding level) and peptide-HLA complex stability (SL stability level). Epitopes were classified as weak binders (WB threshold 500 nM) or strong binders (SB threshold 50 nM) with either weak stability (WS threshold 2 h) or high stability (HS threshold 6 h) according to the predicted scores.
  2. Pre-classified immunodominant peptide candidates are highlighted in italic
  3. aEstimated half-time (t1/2) of dissociation (in hours)
  4. bEstimated peptide binding affinity to HLA alleles as IC50 value (in nM)
  5. cEstimated stability of peptide-MHC I complexes as IC50 value (in nM), and IC50 = half-maximum inhibitory concentration
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Journal of Translational Medicine

ISSN: 1479-5876

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