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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Discovery of immunodominant T-cell epitopes reveals penton protein as a second immunodominant target in human adenovirus infection

Fig. 5

Enrichment of functional A01PentonSTDV- and A02HexonTLLY-specific T cells by IFN-γ-based cytokine secretion assay. HAdV-specific T cells were induced by stimulating PBMCs from healthy donors with A02HexonTLLY, A01PentonSTDV, HAdV5Hexonpp or HAdV5Pentonpp, respectively. Stimulated T cells were isolated using IFN-γ-based cytokine secretion assay. The frequency of IFN-γ-secreting cells among CD3+, CD3+CD4+ and CD3+CD8+ T cells in the CSA fractions before (“Origin”) and after (“Eluate”) enrichment were determined by multicolor flow cytometry. The results of the peptide-specific T-cells enrichment process for weak responders (WR, <0.3 % IFN-γ+CD3+ T cells) and strong responders (SR, >0.3 % IFN-γ+CD3+ T cells) are shown as mean ± standard deviation (SD)

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