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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Discovery of immunodominant T-cell epitopes reveals penton protein as a second immunodominant target in human adenovirus infection

Fig. 2

Screening of HAdV-specific T-cell responses by IFN-γ EliSpot. a IFN-γ EliSpot was performed in 64 healthy donors to evaluate specific T-cell responses to overlapping HAdV hexon (HAdV5Hexonpp) and penton peptide pools (HAdV5Pentonpp), four hexon-derived peptides (A01HexonTDLG, A01HexonTNDQ, A02HexonTLLY, B08HexonDLQD), and four penton-derived peptides (A01PentonSTDV, A02PentonILHT, B08PentnDSKG, B08PentonDSKK). b IFN-γ EliSpot analysis of 26 HAdV-infected HSCT recipients for specific T-cell responses to overlapping HAdV hexon (HAdV5Hexonpp) and penton peptide pools (HAdV5Pentonpp) and to the peptides A01HexonTDLG, A02HexonTLLY and A01PentonSTDV. T-cell responses were assessed in HAdV-infected post-transplant patients. The highest T-cell response is shown for each tested patient. PBMCs cultured with medium alone or in the presence of 1 µg/ml staphylococcal enterotoxin B (SEB) served as negative and positive controls, respectively. IFN-γ EliSpot results are expressed as the number of IFN-γ spots per well (spw). The cut-off value for a positive response was ≥ 5 spw in healthy donors and ≥2 spw in HAdV-infected HCST recipients. Only data and mean frequencies from positive responder are shown. Asterisks indicate statistically significant differences between the antigens (*p < 0.05, **p < 0.01, ***p < 0.001)

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