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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Small cell lung cancer growth is inhibited by miR-342 through its effect of the target gene IA-2

Fig. 1

IA-2 expression is decreased by siRNA. a The knockdown of endogenous IA-2 mRNA by siRNA (determined by quantitative RT-PCR analysis), was reduced ~80 % in NCI-H82. b Western blot shows that protein levels for IA-2 (as evaluated by anti-IA-2 N-terminal #49 antibody), was also significantly reduced. Three major bands of IA-2 are detected as indicated due to post-translational modifications [33]. c IA-2 protein level was reduced ~70 % as determined by the intensity of the 90-kDa bands using the NIH Image J program. d Immunostaining with the IA-2 N-terminal antibody shows that the level of IA-2 in IA-2-specific RNAi treated NCI-H82 cells was significantly lower than the control cells. Data (mean ± SE) were derived from three independent experiments (in triplicate), and normalized to GAPDH mRNA levels. **P < 0.01

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