Fig. 5

The effect of a combination of leptin and atrovastatin in HepG2 cells. a Western blot analysis of LDLR protein levels in HepG2 cells treated with leptin (50 ng/mL) alone or combined with atorastatin (10 μM) for 24 h. b The normalized intensity of LDLR versus GAPDH are presented as the mean ± SD of three independent experiments. c Western blot analysis of PCSK9 protein levels in HepG2 cells treated with leptin (50 ng/ml) alone or combined with atorastatin (10 μM) for 24 h. d The normalized intensity of PCSK9 versus GAPDH are presented as the mean ± SD of three independent experiments. *p < 0.05 represent significant differences compared to the vehicle-treated cells. ^# p < 0.05 represent significant differences compared to the leptin-treated cells. ^& p < 0.05 represent significant differences compared to the atorvastatin-treated cells