Fig. 3

Inhibition of HNF1α expression returned LDLR and PCSK9 expression and LDL uptake during leptin treatment in HepG2 cells. a Western blot analysis of LDLR, PCSK9 and HNF1α protein levels in HepG2 cells transfected with siHNF1α (40 nM) and then treated with leptin (50 ng/mL) for 24 h. b The normalized intensities of LDLR, PCSK9 and HNF1α versus GAPDH are presented as the mean ± SD of three independent experiments. c Representative fluorescence microscopy images of cell-associated Dil-LDL (red), Hoechst-stained nuclei (blue), and the overlay. *p < 0.05 represent significant differences compared to the vehicle-treated cells. ^# p < 0.05 represent significant differences compared to the leptin-treated cells