Fig. 1

The effects of leptin on LDLR and PCSK9 protein levels in HepG2 cells. a Western blot analysis of leptin on LDLR and PCSK9 protein levels in HepG2 cells treated with leptin (0, 5, 25, 50, 100 and ng/mL) for 24 h. bThe normalized intensities of LDLR and PCSK9 versus GAPDH are presented as the mean ± SD of three independent dose-dependent experiments. c Western blot analysis of leptin (50 ng/ml) on LDLR and PCSK9 protein levels in HepG2 cells treated with leptin for 0, 6, 12, 24, 48 h. d The normalized intensities of LDLR and PCSK9 versus GAPDH are presented as the mean ± SD of three independent time-dependent experiments. *p < 0.05 represent significant differences compared to the vehicle-treated cells