Fig. 5

Influence of MPA exposure on receptors involved in CIK cell mediated killing and cell migration. FasL, TRAIL, DNAM, CD11a, CXCR3 and CCR5 were analyzed following short- (24 h), intermediate- (3 days) and long-term (7 days) MPA treatment and all receptors were compared to their individual controls (only 24 h control is displayed). a–c Analyzing receptors involved in CIK cell mediated killing, we observed a stable expression of FasL, TRAIL and DNAM upon short- and intermediate-term MPA treatment. A reduced expression of FasL and TRAIL on T cells and an increased expression of DNAM following long-term MPA exposure was determined (p < 0.05). d–f With regard to CIK cell migration, no alteration in the expression of CD11a, CXCR3 and CCR5 upon short-term MPA exposure was observed. CD11a was downregulated on T cells and upregulated on NK-like T and NK cells following intermediate- and long-term MPA treatment (p < 0.05 and p < 0.01). CXCR3 was downregulated on T cells upon long-term MPA incubation (p < 0.05), whereas CCR5 was expressed to a higher extend on NK-like T cells. n = 5 independent experiments